9.7.2014 SPSFAM Meeting Book

Preferred analytical methods for speciation

Separation of species by chromatography using either  ion chromatography (IC)  or  high performance liquid  chromatography (HPLC) Detection of the separated species by ICP: ICP‐MS is  the most common instrument and ICP‐MS has options  in equipment to obtain MS selectivity to minimize  i t f n er erences

IC‐ICP/MS     or    LC‐ICP/MS

Existing methods for speciation in food

Many papers and methods describing the same type of analysis but the  question is: “Are they robust methods when generalized to other foods?”

A il bl P bli h d th d A i d M S i ti va a e u s e me o s on rsen c an ercury pec a on:

Element 

Method name 

Technique LC-ICP-MS

Published methods US FDA : EAM 4.11 US FDA : EAM 4.10

Arsenic speciation in rice Arsenic speciation in juice

LC-ICP-MS

Arsenic

LC-ICP-MS, Nitric Acid mobile phase LC-ICP-MS, Malonate/ Acetate mobile phase

USGS I‐2193‐02 USGS I‐2192‐02

Arsenic speciation in water

Arsenic speciation in water

Ethylation, CV‐AFS 

Methylmercury in water

US EPA: 1630

HPLC ICP MS ‐ ‐ 

Methylmercury and total mercury in seafood

US FDA: EAM 4 8 .

Extraction, elemental  analysis

Methylmercury in seafood

EU IRMM IMEP-115

Mercury 

Selective extraction

US EPA: 3200

Mercury speciation in soils, sediments

Ethylation, GC‐AFS

USGS OFR_01-445

Methylmercury in water

speciated IDMS

US EPA: 6800

Metal species in water, waste extracts