9.7.2014 SPSFAM Meeting Book

Protein Determination

1

Sentandreu et al. , 2010 , 9 , 3374‐3383,  J. Proteome Res.

SDS‐PAGE protein profile

Trypsin digestion‐derived d pepti e sequence

2

DQGTFEDFVEGLR EAFLLFDRTGDAK ILGNPSKEEMNAK ALGQNPTNAEINK MTEEEVEELMK EEMNAKK

Myosinlightchain3

3 Fragmentation pattern

F

F

C

F

C

C

F: fresh;C: cooked

4

Absolute quantitation by LC‐MS/MS 

= 84.02 min

t R

100

Relative abundance

0

Time (min)

Meat Species Substitution - Proteomics

+ High discriminating power ► Within-species tissue differentiation Extraction protocols ‘easy’ to standardize Primary amino acid sequence of peptides resistant to processing

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‐ High cost of modern mass spectrometry equipments Need for skilled operators

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