OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp
67 71 70 67 73
68.5
20
C1
M1
B1
2.12 3.10
72.5
30
C1
M1
B1
0.71 0.98
Biofish Electrode Stability
80
60
40
20
0
Biofish Results (ppm)
0
5
10
15
20
25
30
35
Time (days)
Figure 13. 30-day stability plot
Robustness
(a) Methodology.- Three method parameters were varied including extraction volume, ultraturrax time, and extract storage time before analysis. The parameter values are shown in Table 15 and Table 16 shows the factorial design employed. Treatment combination 9 uses the normal method parameter values. Treatment combinations 1-8 use either the high or low values for each parameter. For each treatment combination, 2 replicates of Incurred raw shrimp head off with 50-100 ppm sulfite (measured as SO 2 ) were analyzed.
(b) Results.- The results of the duplicate analyses are shown in Table 16. The factorial design was translated into the Youden design in Table 17 and the Youden test was applied (Table 18).
Table 15. Robustness parameters RAW SHRIMP HEAD OFF ROBUSTNESS PARAMETERS
VALUE 1
VALUE 2
VALUE 3
A. EXTRACTION VOLUME B. ULTRATURRAX TIME
16 mL 10 sec
18 mL 20 sec
20 mL 30 sec
C. EXTRACT STORAGE BEFORE ANALYSIS
none
2 h at 25°C
4 h at 25°C
29
Made with FlippingBook - Online magazine maker