OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp
LOD and LOQ were estimated as in the method developer study. The estimated values are presented in Table 21. The tested matrix yielded estimated LOQ values lower than 20 mg/kg for both method ranges.
Table 20. Results obtained by the independent laboratory (all sulfite results expressed in mg/kg)
Experiment Date: 9/21/2017
Experiment Date: 9/22/2017
Sample 4
Sample 1
Sample 5
Sample 2 Sample 3
Portion 1
32
74
112
Portion 1
<10
237
Portion 2
35
73
99
Portion 2
<10
238
Portion 3
33
76
108
Portion 3
<10
244
Monier- Williams Method
Monier- Williams Method
Mean
33
74
106
Mean
-
240
% RSD r
6.1
1.8
6.3
% RSD r
-
1.6
Theoretical level SO2 (mg/kg)
Theoretical level SO2 (mg/kg)
54
107
160
0
317
Recovery ( %)
61
69
66
-
76
Recovery ( %) (Mean /theoretical level)*100
(Mean MW
/theoretical
MW
level)*100
Portion 1
50
97
145
Portion 1
0
253
Portion 2
55
101
145
Portion 2
0
242
Biofish method (30-150 mg/kg range)
Biofish method (30-150 mg/kg range)
Portion 3
55
105
144
Portion 3
0
251
Portion 4
54
105
142
Portion 4
0
249
Portion 5
52
103
141
Portion 5
0
245
Mean
53
102
143
Mean
0
248
R=0.9999 m=- 219486.84 b=-1.53
R=1 m=- 221557.00 b=-0.01
s r
2.2
3.3
1.8
s r
-
4.5
% RSD r
4.1
3.3
1.3
% RSD r
-
1.8
Accuracy (%)
Accuracy (%)
160
138
135
-
103
(mean Biofish
/mean OMA
)*100
(mean Biofish
/mean OMA
)*100
33
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