OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

The Linearity and Matrix studies demonstrated linear dose responses from 0 (non-detect) to 300 mg/kg sulfite. As a whole, accuracy, recovery and bias within range results indicate the kit provides accurate and precise sulfite quantification for all the evaluated matrices, confirming that sample preparation and assay procedures produce acceptable results. The analyses of reference material has shown that the results obtained for sulfite were adequate in peeled shrimp. Data produced in the Lot –to –Lot Consistency and Stability study show consistent results for calibration and measurement kit components during 6 months in dry storage conditions and during 30 days for the Biotest electrodes. The robustness study showed that variation of the extract volume and ultraturrax time produced unacceptable results. The package insert defines the correct protocols; the procedure must be performed according to these instructions to avoid mistakes. The results obtained in the study were similar to those performed by the independent laboratory. The analysis performed by the independent laboratory with fortified shrimp showed a very good accuracy and precision. The LOQ was lower than 20 ppm for both detection ranges of the method. From the independent laboratory point of view, the Biofish system was fast and convenient to carry out, and demonstrated precision, excellent linearity and consistency across the two detection ranges.

Conclusion

In summary, the Biofish-300 SUL method is precise and accurate in all three evaluated shrimp matrices. The LOQ was established at 30 mg/kg for the evaluated matrices. The method is selective, robust, consistent and stable in most of the studied circumstances.

References

(1) COMMISSION DECISION of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (2) REGULATION (EC) No 1333/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on food additives. (3) AOAC Official method. 990.28. Sulfites in Foods. Optimized Monier – Williams method. J. AOAC Int. , 47.3.43 , 20.123-20.135 (4) Youden, W.J & Steiner, E.H.; «Statistical Manual of the AOAC–Association of Official Analytical Chemists», AOAC-I, Washington DC: 1975, p. 35 ff. (5) www.fapas.com

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