OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp
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Limit of Quantitation (LOQ) .—3 x LOD
Principle of the Method BIOFISH 300 SUL method is an enzymatic amperometric biosensor for the quantification of
, in crustaceans. A biosensor is defined as an analytical device that comprises a
sulfites, expressed as SO 2
biological recognition element and a transducer component. Biolan’s biosensors are composed of the
BIOFISFH 300, a potentiostat with an electrochemical cell where the reaction takes place as the transducer
element, and the Biotest, the working electrode that contains a redox enzyme, together with other
electroactive components, that interacts with the analyte.
The analysis is performed by first extracting the sulfites from the matrix, by a simple
homogenization of a representative blended sample with an Extraction Solution, followed by the addition
of the extracted solution into the cuvette of the device. The reaction occurs when the sulfite molecules
reach the Biotest’s surface and are oxidized by the enzyme, releasing electrons, and thus, increasing the
electric current inside the electrochemical cell. This change in the electric current is then correlated to the
SO 2 content by a previous calibration of the device with known sulfite standards.
General Information Sulfites are some of the oldest and most widespread preservatives in our food supply. They are
food additives that have antioxidant properties, but they are also recorded as allergens by the main
international regulatory bodies on food safety because of their adverse health effects. Reactions to
sulfites can range from mild to life-threatening. Sulfite sensitive people may have a similar reaction as
those with a food allergy. Sulfites can trigger asthma and symptoms of an anaphylactic reaction and many
people who have asthma may also have a sulfite sensitivity.
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