OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

2.9 Analysis of QC Test Portion Sets 2.9.1 The Coordinating Laboratory will retain two Quality Control Test Portion Sets. These sets are identical to the collaborator test portion sets and will be randomized and blind coded. 2.9.2 QC Set 1 will be analyzed on the day of shipment to check the sulfite levels. These data are used only to gauge whether the target levels will be met in the study. 2.9.3 QC Set 2 will be the “official” test portion set analyzed by the Coordinating Laboratory on the day the collaborators initiate analyses and will follow section 2.7 above. 2.10 Reporting Raw Data 2.10.1 Data will be reported using the data report form in Appendix 3.5. 2.10.2 Each collaborator will complete the data report form and email it to the co- Study Director as indicated on the form. 2.10.3 Each collaborator or collaboration site must retain all related test results for a minimum of one year. 2.10.4 Collaborators may also submit a comment form (Appendix 3.4). 2.10.5 A table reporting all raw data from all collaborators must be included in the collaborative study report. All submitted collaborator comments must be included in the collaborative study report. 2.11 Analyzing Raw Data 2.11.1 The Study Director will analyze the study data once all data report forms have been received. 2.11.2 Construct a Youden plot of replicate pairs by plotting first replicate versus second replicate for each material. Usually major discrepancies will be apparent. 2.11.3 Access the “AOAC Interlaboratory Study Workbook – Blind (Unpaired) Replicates” for statistical analyses: http://www.aoac.org/AOAC_Prod_Imis/AOAC/SD/SGR/AOAC_Member/SDCF/S DGRCF/SDSGM.aspx?hkey=086fdba4-32e2-43f0-8272-7d41a6603e87 2.11.4 Analyze each material and each method separately. Look for significant findings from Cochran’s and Grubbs’ outlier tests. 2.11.5 Data may be excluded due to an assignable cause if sufficient justification is provided. Data may not be excluded on a statistical basis only. 2.11.6 Using the valid data, report the following parameters for each material and each method: 2.11.6.1 Mean Concentration .—Mean candidate method result across all collaborators.

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