AOAC SPIFAN Nutrients ERP-Final Review (Dec. 2018)
2015.10 (Dec. 2018) Carn-07 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE
Appendix II. Standard Method performance requirements (SMPRs) Method Performance Requirements for Total Choline (SMPR 2012.013)
Analytical range
2-250 a
Limit of detection (LOD) Limit of quantitation (LOQ) Repeatability (RSD r )
0.7 a 2.0 a
2 a
≤10%
20 a
100 a 200 a
≤5%
Recovery
90 to 110% of mean spiked recovery over the range of the assay
Reproducibility (RSD R )
2 a
≤15%
20 a
≤10% 100 a 200 a a mg/100 g Concentrations apply to: (1) “ready-to-feed” liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. For all concentrations, choline will be expressed as mg/100 g reconstituted liquids. Report as total choline.
Method Performance Requirements a for L-Carnitine (SMPR 2012.010)
Analytical range
0.16-20 b ≤0.16 b
Limit of quantitation (LOQ) Repeatability (RSD r )
0.16-20 b
≤8%
Recovery
90 to 110% of mean spiked recovery over the range of the assay
Reproducibility (RSD R ) ≤ 15% a Concentrations apply to: (1) “ready-to-feed” liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. b mg/100 g reconstituted final product. 0.16-20 b
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