AOAC SPIFAN Nutrients ERP-Final Review (Dec. 2018)
Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE
Table 2. Method Performance Requirements for Total Choline (SMPR 2012.013)
2-250 a
Analytical range
0.7 a 2.0 a
Limit of detection (LOD) Limit of quantitation Repeatability (RSD r )
2 a
≤10% ≤5%
20 a 100 a 200 a
Recovery
90 to 110% of mean spiked recovery over the range of the assay
2 a
Reproducibility (RSD R )
≤15% ≤10%
20 a 100 a 200 a
a mg/100 g Concentrations apply to: (1) “ready-to-feed’ liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. For all concentrations, choline will be expressed as mg/100 g reconstituted liquids. Report as total choline.
Table 3. Method Performance Requirements for L-Carnitine (SMPR 2012.010)
0.16-20 b ≤0.16 b
Analytical range
Limit of quantitation (LOQ) Repeatability (RSD r )
0.16-20 b
≤8%
Recovery
90 to 110% of mean spiked recovery over the range of the assay
Reproducibility (RSD R ) ≤ 15% a Concentrations apply to: (1) “ready-to-feed’ liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. b mg/100 g reconstituted final product. 0.16-20 b
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