AOAC SPIFAN Nutrients ERP-Final Review (Dec. 2018)
Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE
Limit of Quantitation (LOQ) The Limit of Quantitation for the analysis was determined through back calculation of the lowest calibration standards of 10 ng/mL through the default weights and dilution factors. A concentration of 0.05 mg/100g for carnitine and choline in both analyses can be achieved. The LOQ required by the SMPR for total choline is 2.0 mg/100g and ≤ 0.16 mg/100g for carnitine. The free and total analyses were both tested at these levels in the elemental infant formula placebo to verify the method can quantitate at these levels with precision and accuracy (Tables 10 and 11).
Table 10. LOQ Free Analysis
Choline Day/
Fortification (mg/100g)
Innate
Marginal Recovery
MEAN
SD (%) 5.7
RSD r
Replicate
(mg/100g)
(%)
(%)
(%) 6.1
1/1 1/2 2/1 2/2 3/1 3/2
1.5 1.5 1.5 1.5 1.5 1.5
0.118 0.118 0.118 0.118 0.118 0.118 MEAN
89.9 97.9 94.8 90.7
93.9
92.8
2.9
3.1
100.0 100.3
100.2
0.2
0.2
95.6
3.9
Overall RSD r
Carnitine Day/ Replicate
Fortification (mg/100g)
Innate
Marginal Recovery
MEAN
SD (%) 0.6
RSD r
(mg/100g)
(%)
(%)
(%) 0.6
1/1 1/2 2/1 2/2 3/1 3/2
0.16 0.16 0.16 0.16 0.16 0.16
102.0 101.2 101.6 95.7 101.1 7.6 7.5 106.4 102.9 106.7 102.5 104.8 2.7 2.6 4.6 Overall RSD r
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