AOAC-RI ERP Book MICRO Sept 2016.pdf
February 20, 2013
Figure 1. 3M Petrifilm Salmonella vs. USDA – raw ground chicken, raw ground beef, raw ground pork, chicken nuggets, and 1 pasteurized liquid whole eggs.
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Estimate total aerobic counts
Screen for Indigenous Salmonella
Matrix
3M
USDA
Raw ground chicken, pork, beef : 25g + 225mL* Liquid egg : 100g + 900 mL* Cooked chicken nuggets : 325g + 2925mL* Homogenize 2 min 18-24h at 41.5±1°C *3M Salmonella Enrichment + Supplement (prewarmed to 41.5C)
Ground chicken, pork, beef: 25g + 225mL* Liquid egg: 100g + 900 mL* Cooked chicken nuggets : 325g + 2925mL* Homogenize 2 min *BPW
MPN
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Egg
Liquid eggs 18-24h h at 35±2°C
Ground chicken 20-24h h at 35±2°C
Chicken, pork, beef Transfer 0.1 mL BPW
0.1ml sample + 10ml mRV 0.5ml sample + 10ml TT (Hanja) 22-24h at 42 ± 0.5°C
to 9.9 mL RV10 8 h at 41.5±1°C
0.1ml sample + 10ml mRV 0.5ml sample + 10ml TT (Hanja) 22-24h at 42 ± 0.5°C
• Streak to XLT4 (or DMLIA) & BGS Incubate at 35±2°C Examine at 18-24h & 42-48h
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Run the 3M Petrifilm Salmonella Express System
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• Streak to XLT4 (or DMLIA) & BGS Incubate at 35±2°C Examine at 18-24h & 42-48h
Transfer typical colonies to TSI/LIA slants 24±2h at 35°C
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Pick typical colony and transfer to TSI/LIA slants 24±2h at 35°C
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Transfer typical colonies to TSI/ LIA slants 24±2h at 35±2°C
Biochemical/serological confirmation on all enrichments according to USDA/FSIS
Biochemical/serological confirmation on all enrichments according to USDA/FSIS
Biochemical/serological confirmations on all enrichments according to USDA/FSIS
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