AOAC-RI ERP Book MICRO Sept 2016.pdf
Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report
Table 21. 3M Petrifilm SALX Method Comparison POD Results for Raw Frozen Shrimp (Internal Data) 1
3M Petrifilm SALX Method
Reference Method
x c
Strain MPN a /25g
N b
95% CI g
e
d
dPOD CP f
95% CI
95% CI
x
Matrix
18 Hr Primary Enrichment – Time Point
POD CC
POD CP
Frozen Uncooked Shrimp -
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
Salmonella
8 Hr 2 0 RV-R10 Enrichment Virchow
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
Confirmed by the 3M Confirmation Disk
18 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 8 Hr 2 0 RV- R10 Enrichment 24 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 18 Hr Primary Enrichment – 18 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 8 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
ATCC
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
51955
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
Frozen Uncooked Shrimp -
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
Salmonella
8 Hr 2 0 RV-R10 Enrichment Virchow
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
Confirmed by the Reference Method
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
ATCC
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
51955
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
0.77 (0.41- 1.36)
20
12
0.6 0.39 – 0.78 12
0.6 0.39 – 0.78
0 -0.28 – 0.28
a MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator with 95% confidence interval b N = Number of test potions c x = Number of positive test portions
d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the candidate method confirmed results and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
2
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