AOAC-RI ERP Book MICRO Sept 2016.pdf
Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report
Table 23. 3M Petrifilm SALX Method Comparison POD results for Raw Ground Beef (Internal Data) 1
3M Petrifilm SALX Method
Reference Method
X c
Strain MPN a /25g
N b
95% CI g
e
d
dPOD C f
95% CI
95% CI
x
Matrix Time Point
POD R
POD C
18 Hr Primary Enrichment – 8 Hr 2 0 RV-R10 Enrichment 18 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 8 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment
Raw Ground Beef
Salmonella
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
Vs. USDA/FSIS- MLG
1.07 (0.65- 1.79)
Ohio
20
13
0.65 0.43 – 0.82 13
0.65 0.43 – 0.82
0 -0.28 – 0.28
STS 81
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
1.07 (0.65- 1.79)
20
13
0.65 0.43 – 0.82 13
0.65 0.43 – 0.82
0 -0.28 – 0.28
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
1.07 (0.65- 1.79)
20
13
0.65 0.43 – 0.82 13
0.65 0.43 – 0.82
0 -0.28 – 0.28
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
1.07 (0.65- 1.79)
20
13
0.65 0.43 – 0.82 13
0.65 0.43 – 0.82
0 -0.28 – 0.28
a MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator with 95% confidence interval b N = Number of test potions c x = Number of positive test portions
d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the candidate method confirmed results and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
2
Made with FlippingBook