ClearLLab LS CaseBook

ThisCD34/CD5dot plot is gatedonCD45+ events. Unlike thedot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). As noted elsewhere in the analysis, the blasts (pink) express CD34 but not CD5. CD5 positive T lymphocytes (red) are also present.

Results of Flow Cytometric Immunophenotyping

Flow cytometric immunophenotyping identifies a phenotypically distinct population of cells with increased light scatter properties that express CD10, CD19, partial CD20, partial CD34 and low density CD45. Surface immunoglobulin light chain expression is absent. Taken together, the results are consistent with B acute lymphoblastic leukemia/lymphoblastic lymphoma. Correlation with clinical and laboratory data is also recommended.

Neoplastic Process of B cell Origin > B lymphoblastic leukemia/lymphoblastic lymphoma > Case #4: B Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma

Every Event Matters

37 Beckman Coulter • ClearLLab LS Casebook