ClearLLab LS CaseBook

This CD34/CD20 dot plot is gated on CD45+ events. Unlike the dot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). No co-expression of CD20 and CD34 is noted. The blue events noted on the diagonal are consistent with high background fluorescence.

ThisCD34/CD5dot plot is gatedonCD45+ events. Unlike thedot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). No co-expressionof CD34andCD5 is noted. The higher densityCD5 T lymphocytes and lower density CD5 aberrant B lymphocytes are distinguishable here. The blue events noted on the diagonal are consistent with high background fluorescence.

Results of Flow Cytometric Immunophenotyping

Flowcytometric immunophenotyping identifies a phenotypically distinct population of cellswith low light scatter properties that express CD19, low density CD20, CD5, and CD45 and display Lambda immunoglobulin light chain restriction. Taken together, the findings in this case are most consistent with chronic lymphocytic leukemia/small lymphocytic lymphoma. Note that correlationwith clinical and laboratorydata is recommended, and that additional immunophenotyping may be warranted.

NeoplasticProcessofBcellOrigin > Chronic lymphocytic leukemia/small lymphocytic lymphoma > Case#6:ChronicLymphocyticLeukemia/SmallLymphocyticLymphomaLymphocyticLymphoma

Every Event Matters

53 Beckman Coulter • ClearLLab LS Casebook