ClearLLab LS CaseBook

This CD20/Side Scatter dot plot is gated on CD45+ events. Unlike the dot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). Clear delineation between the CD20 positive (B lymphocytes) and CD20 negative (T lymphocytes) is not possible.

ThisCD56/CD19dot plot isgatedonCD45+events. Unlike thedot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), andblasts (pink). BothB lymphocytes andNK cells are present. The blue events noted on the diagonal are consistent with high background fluorescence.

This CD34/CD10 dot plot is gated on CD45+ events. Unlike the dot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). A distinct population of CD10 positive, CD34 negative events corresponds to aberrant B lymphocytes (red). The blue events that appear to co-express CD34 and CD10 are consistent with high background fluorescence.

This CD34/CD20 dot plot is gated on CD45+ events. Unlike the dot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). The blue events are consistent with debris.

Neoplastic Process of B cell Origin > Follicular lymphoma > Case #8: Follicular Lymphoma

Every Event Matters

68 Beckman Coulter • ClearLLab LS Casebook