ClearLLab LS CaseBook

This CD45/Side Scatter dot plot permits distinction of various cell populations according to a combination of CD45 antigen density and side scatter. The usual populations include lymphocytes (red), monocytes (green), and granulocytes (blue). Additionally, the “Blast” gate (pink) has been used to define tightly clustered population of mononuclear cells in this case. Note that the label for the Blast gate is not immediately adjacent to it, unlike normal lymphocytes, monocytes, and granulocytes, blasts and other phenotypically aberrant populations do not conform to any predictable pattern. A CD45+ gate may be used to exclude CD45 negative debris but these events should not be ignored when analyzing a case, as some aberrant populations are CD45 negative. The gates should be adjusted such that they conform to the naturally occurring separations among the populations, but where no separation is observed an estimate based on experiencemay be used. In this case themonocyte gate is not being used and has been pushed to the side. The granulocyte gate is in its usual position, but the events within it do not in fact appear to be granulocytes.

Neoplastic Process of T Cell Origin > T lymphoblastic leukemia/lymphoblastic lymphoma > Case #9: T Lymphoblastic Leukemia/T Lymphoblastic Lymphoma

Every Event Matters

72 Beckman Coulter • ClearLLab LS Casebook