ClearLLab LS CaseBook

This CD20/Side Scatter dot plot is gated on CD45+ events. Unlike the dot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). The majority of the blasts (pink) do not express CD20, and those that do may represent hematogones.

ThisCD56/CD19dot plot isgatedonCD45+events. Unlike thedot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). The majority of the blasts are CD56 and CD19 dual negative, with the small subset of CD19 positive pink events being consistent with B lymphocytes and/or hematogones. NK cells (red, CD56 positive, CD19 negative) are alsopresent. The blue and red events noted on the diagonal are consistent with high background fluorescence.

ThisCD34/CD10dot plot isgatedonCD45+events. Unlike thedot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). As noted elsewhere the blasts (pink) express CD34. The blue and red events noted on the diagonal are consistent with high background fluorescence.

This CD34/CD20 dot plot is gated on CD45+ events. Unlike the dot plots gated on Lymphocytes, it includes the named populations defined on the CD45/Side Scatter dot plot: lymphocytes (red), monocytes (green), granulocytes (blue), and blasts (pink). As noted elsewhere the blasts (pink) express CD34. They do not express CD20. The blue and red events noted on the diagonal are consistent with high background fluorescence.

Neoplastic Process of T Cell Origin > T lymphoblastic leukemia/lymphoblastic lymphoma > Case #9: T Lymphoblastic Leukemia/T Lymphoblastic Lymphoma

Every Event Matters

77 Beckman Coulter • ClearLLab LS Casebook