ClearLLab LS CaseBook

This CD20/CD19 dot plot is gated on Lymphocytes. The B lymphocytes display expected normal co-expression of CD19 and CD20. The aberrant population is negative for both CD19 and CD20.

This CD34/CD10dot plot is gated on Lymphocytes. Themajority of events, however, express high density CD34, indicating that they are not, in fact, lymphocytes but instead represent blasts. Although blasts are generally colored pink in ClearLLab LS analysis, the overlap in CD45 antigen density and light scatter properties between normal lymphocytes and the aberrant population in this case results in their being included in the Lymphocytes gate.

This CD19/CD10 dot plot is gated on Lymphocytes. A small number of B lymphocytes is present in this sample. The aberrant population is negative for both CD19 and CD10.

This CD5/CD19 dot plot is gated on Lymphocytes. Normal B lymphocytes arepresent. Thepresence of normal T lymphocytes is obscured by the overlapping aberrant population which displays similarly high density CD5 expression.

Neoplastic Process of T Cell Origin > T lymphoblastic leukemia/lymphoblastic lymphoma > Case #10: T Lymphoblastic Leukemia/T Lymphoblastic Lymphoma

Every Event Matters

83 Beckman Coulter • ClearLLab LS Casebook