Total Collaborative Study Protocol_Solus One Salmonella v1 1
( c ) Positive Control (Red Label) .— 3 mL or 10 mL in working dilution. Contains heat‐killed
Salmonella Agona in diluent with preservative. The positive control is black in color. Store at 2–8°C.
( d ) Conjugate (Orange Label) .— 11 mL or 60 mL in working dilution. Contains horseradish
peroxidase‐antibody conjugate in diluent with preservative. Store at 2–8°C.
( e ) Substrate (Blue Label) .— 11 mL or 60 mL in working dilution. Contains 3,3’,5,5’‐
Tetramethylbenzidine (TMB), hydrogen peroxide and stabilizers. Note: Solution should be colorless.
Store at 2–8°C.
( f ) Stop Solution (Silver Label) .— 11 mL or 60 mL in working dilution and contains 0.2M sulfuric
acid. Store at 2–8°C.
( g ) Washing Buffer Concentrate (25x) .— 6 x 10 mL or 5 x 60 mL. Store at 2–8°C.
( h ) Washing Buffer Activator .— 6 x 1 Sachet or 5 x 1 Sachet. Store at 2–8°C.
( i )
Product Instructions .
Additional supplies and reagents
( a ) Dehydrated Buffered Peptone Water (BPW) Medium according to ISO 6579, CAT No. MED017 . ( b ) Dehydrated Solus modified Buffered Peptone Water (Solus mBPW), CAT No. MED038 . ( c ) Solus One Salmonella Supplement , CAT No. SALSUPP‐22.5 & SALSUPP‐112 . ( d ) Dynex Technologies DS2 Instrument (Dynex DS2) . — Dynex Technologies, Chantilly, VA.
( e ) Computer with DS2 Software . ( f )
Dynex Technologies 300 µL Sample Tips .
( g )
Dynex Technologies 1300 µL Reagent Tips .
( h ) Sample Tubes .— 12 mm external diameter and 75 mm height.
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