Total Collaborative Study Protocol_Solus One Salmonella v1 1
Polypropylene Tubes .— 2 mL and 15 mL and 25 mL.
( i )
( j ) Frit filters .— Optional for matrices with a lot of particulate , CAT No. FRI001.
( k ) 70% v:v Ethanol .— For preparation of the Solus One Salmonella Supplement.
( l )
Deionized water .
( m ) Sterile collection sponge for sampling environmental surfaces .— World Bioproducts EZ Reach
Sponge Sampler with Hi Cap Neutralizing Buffer, or equivalent.
( n ) Sterile collection swab for sample environmental surfaces . ( o ) Letheen Broth neutralizing buffer .
Apparatus
( a ) Refrigerator .— capable of maintaining 2–8°C.
( b ) Incubators .— capable of maintaining 37 ± 1°C and 41.5 ± 1°C.
( c ) Circulating water bath .— capable of maintaining 42–50°C.
( d ) Heating block or water bath .— capable of maintaining 85–100°C.
( e ) Autoclave for decontamination of samples . ( f ) Homogenizer (g) Measuring cylinder .— capable of measuring 2 L.
( h ) Vessel or container .— capable of storing 2 L.
( i )
Filter laboratory blender bags .
( j ) Sterile collection sponge and swabs for sampling environmental surfaces . ( k ) Serological Pipette Bulbs (Automatic Pipette) .— For sampling and delivering of 1–10 mL.
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