Total Collaborative Study Protocol_Solus One Salmonella v1 1
( c ) Start heating block or heat water bath to 85–100°C prior to initiating testing. ( d ) Allow heated samples to cool to room temperature (15–25°C). This may be accelerated by placing the test tubes in cold water for 5 min. ( e ) Where appropriate frit filters will be used with denatured matrices that contain high concentrations of particulates thereby minimizing sample handling errors. ( f ) Change pipette tips in between samples.
Pathogen Detection (Automated Sample Preparation Method)
( a ) Equilibrate test kit to room temperature (15–25°C) an hour before use. ( b ) Start the Dynex DS2 and allow instrument to conduct the ‘Self‐Test’. ( c ) Load required materials: pipette tips, reagents, washing buffer, etc. ( d ) Empty waste. ( e ) Start heating block or heat water to 85–100°C prior to initiating testing. ( f ) Allow heated samples to cool to room temperature (15–25°C). This may be accelerated by
placing the test tubes in cold water for 5 min.
( g ) Where appropriate use frit filters with denatured matrices that contain high concentrations of
particulates to minimize sample handling errors.
Sample Enrichment and Preparation
( a ) Raw Beef Trim (375 g test portions)
Pre‐warm 1,125 mL of Solus BPW (ISO) Medium supplemented with Solus Salmonella supplement
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