Total Collaborative Study Protocol_Solus One Salmonella v1 1
and select the “Report” button.
2) The report can be viewed and printed from the report display screen.
3) The results are displayed in a 96 well format with each individual optical density reading.
4) A secondary file which can be used to export the results into a LIMS system is created in a
folder on the desk top called “ELISA Export Results”, this is in a CSV (comma separated
values) file format.
Immunoassay result interpretation
The presence or absence of the pathogen is expressed as optical density (OD 450
) measurements
using the Dynex DS2 instrument plate reader or a microplate reader with a 450 nm filter where a Dynex
< 0.100 is valid
DS2 instrument is unavailable. For control assay acceptance criteria, a result of an OD 450
for a Negative Control, whereas a result of an OD 450
> 0.500 is valid for a Positive Control. A result of an
OD 450 < 0.200 is considered to be negative for Salmonella . A result of an OD 450
≥ 0.200 is considered
presumptive positive for Salmonella .
Confirmation
All samples are confirmed according to the appropriate reference methods specified for the matrix
and analyte.
Validation Study
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