Total Collaborative Study Protocol_Solus One Salmonella v1 1
presence of additional selective pressures from higher supplement levels could result in the generation
of false negative results. As such, the enrichment medium used with these matrices was modified to be
less stringent than the original (2.22 mL Solus One Salmonella supplement per 1.0 L medium compared
to 4.44 mL of supplement per 1.0 L), a subset of inclusivity and exclusivity strains was also tested.
Independent Laboratory Studies
Inclusivity/Exclusivity Study
This study was carried out with pure cultures to examine the ability of the Solus One Salmonella
method to detect a variety of claimed Salmonella strains and to distinguish those strains from non‐
Salmonella strains.
In addition, at least two serovars were selected from Salmonella enterica subspecies salamae ,
Salmonella enterica subspecies arizonae , Salmonella enterica subspecies diarizonae , Salmonella enterica
subspecies houtenae and Salmonella enterica subspecies indica . Also at least two Salmonella bongori
strains and only one strain per serovar of Salmonella enterica subspecies enterica were selected.
For the inclusivity study evaluation of Solus One Salmonella , 59 Salmonella strains were cultured in 9
mL of Solus mBPW Medium supplemented with 2.22 mL Solus One Salmonella supplement per 1.0 L
medium for 16–20 h at 41.5 ± 1°C. After incubation, the culture tubes were diluted with sterile Solus
supplemented mBPW enrichment medium to 10–100x the Limit of Detection (LOD 50
) of the method,
approximately 1 x 10 4 – 1 x 10 5 CFU/mL.
For the exclusivity portion of the evaluation, 10 species including Gram negative bacterial species
and Gram positive bacterial species, were cultured in 9 mL of Brain Heart Infusion (BHI) broth for 22 ± 2
Page 23 of 39
Made with FlippingBook - Online Brochure Maker