Total Collaborative Study Protocol_Solus One Salmonella v1 1

1  Collaborators will be provided with enough test material to test each method (Solus One  Salmonella and  2  FDA BAM Ch. 5). Collaborators will receive two sets of test portions: 36 x 375 g (Solus One  Salmonella )  3  and 36 x 25 g (FDA BAM). One temperature control sample will also be included in the shipment to  4  document the temperature of the sample upon receipt. One additional 30 g control (uncontaminated)  5  test portion will be provided to each collaborator to determine the total plate count on the day of test  6  initiation. Record all results on the data sheets provided. See Attachment 5 for data sheets. Please  7  review both methods carefully before initiating analysis. 

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Reference  Pre‐enrichment 

Food Type 

Reference  FDA BAM a

Solus One  Salmonella

NFDM  BPW + supplement a https://www.fda.gov/food/laboratory‐methods‐food/bacteriological‐analytical‐manual‐bam‐ 12  13  14  A brief description of the methods is listed here. Follow the Solus One  Salmonella  kit insert and FDA  15  BAM Ch. 5 instructions when conducting the experiments.   Brilliant Green water  11  chapter‐5‐salmonella

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1. 

Solus One  Salmonella

1.1  To each 375 g NFDM portion, homogenize (2 min) in 1125 mL of supplemented BPW 

(pre‐warmed to 37°C) and incubate portions at 41.5 ± 1°C for 21–22 h.

1.2  When incubation is complete, carefully remove a 1 mL aliquot, avoiding particulate and  fatty matter. Heat aliquot to 85–100°C for 15–20 min. Allow to cool to room  temperature (20–25°C), or place test tubes in cold water for 5 min. Keep the unheated 

samples for confirmation. Samples can be stored up to 72 h at 2–8°C.

1.3  Prior to analysis, bring the entire contents of the kit to room temperature (15–25°C) by 

placing the kit on a sanitized bench top for 1 h prior to use. 

1.4  Determine the number of wells required for the test. Take the required number of strips  from the pouch and fit them to the frame provided. Note: Unused strips should be 

returned to the pouch and stored at 2–8°C. 

1.5  Dynex DS2 Automated procedure : Turn on power to the Dynex DS2 instrument,  followed by the power to the computer. Open the Matrix software on the computer and  enter login information. The Dynex DS2 instrument will perform a self‐diagnostic check.  1.6  In the DS‐ Matrix software, select “File”, “Worklist Editor”. Then select “New Sample 

Batch”. 

1.7  1.8  1.9 

Enter your sample batch ID as required.  Enter the total number of samples in the batch. 

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Sample ID may then be typed into the empty positions in the rack or assigned 

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