Total Collaborative Study Protocol_Solus One Salmonella v1 1
Appendix 10.2 Study Materials 1 2 Materials provided by Solus Scientific, Sponsor Laboratory 5 6 Materials provided by Q Laboratories, Inc., Coordinating Laboratory 7 8 1. Standard Methods Agar for background APC of NFDM 9 10 Enrichment 11 2. BPW 12 3. Brilliant Green Dye 13 4. Individual Ingredients to prepare FDA RV 14 5. TT Broth for FDA reference method 3 4 1. Solus One Salmonella test kits
15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32
Selective agar
6. Hektoen Agar
7. XLD Agar
8. Bismuth Sulfite
Confirmation
9. TSI 10. LIA 11. BHI
12. API 20E or VITEK GN cards 13. O and H serotyping supplies
Additional Supplies and Reagents required but not provided
14. Refrigerator, capable of maintaining 2–8°C
15. Incubator, capable of maintaining 37 ± 1°C and 41.5 ± 1°C 16. Circulating water bath, capable of maintaining 42–50°C 33 17. Heating block or water bath, capable of maintaining 85–100°C 34 18. Autoclave for decontamination of samples 35 19. Homogenizer 36 20. Measuring cylinder , capable of measuring 2 L 37 21. Vessel or container , capable of storing 2 L 38 22. Serological Pipette Bulbs (Automatic Pipette) , for sampling and delivering of 1–10 mL 39 23. Serological pipettes , a erosol resistant 40 24. Precision pipettes , f or sampling and delivering of 100–300 µL, and 200–1,000 µL 41
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