Total Collaborative Study Protocol_Solus One Salmonella v1 1
Romaine lettuce (bagged), NFDM (powder) and selected environmental surfaces. In addition,
performance is equivalent to that of U.S. Department of Agriculture Food Safety and Inspection Service
Microbiology Laboratory Manual , (MLG) 4.09 (January 2017) Isolation and Identification of Salmonella
from Meat, Poultry, Pasteurized Egg, and Siluriformes (Fish) Products and Carcass and Environmental
Sponges (2) for detection of Salmonella spp. from raw beef (trim) and pasteurized eggs.
Definitions
( a ) Probability of Detection.— Probability of Detection (POD) is t he proportion of positive analytical outcomes for a qualitative method for a given matrix at a given analyte level or concentration.
POD is concentration dependent.
( b ) Difference of Probabilities of Detection .— Difference of probabilities of Detection (dPOD) is the difference between any two POD values. If the confidence interval of a dPOD does not contain zero,
then the difference is statistically significant at the 5% level (3).
Principle of the Method
Solus One Salmonella is an antibody‐based high sensitivity ELISA method paired with media and our
proprietary media supplement – Solus One Salmonella supplement; for the rapid and specific detection
of Salmonella species in select foods and environmental samples. Solus One Salmonella relies on
antibodies attached to the wells of microplate strips by non‐covalent biological interactions that are
highly specific to Salmonella antigens. Samples are heat treated and an aliquot is added to the antibody
coated wells.
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