Total Collaborative Study Protocol_Solus One Salmonella v1 1
( c ) Dynex Technologies DS2 Instrument (Dynex DS2) . — Dynex Technologies, Chantilly, VA.
( d ) Computer with DS2 Software . ( e ) Dynex Technologies 300 µL Sample Tips . ( f ) Dynex Technologies 1300 µL Reagent Tips .
( g ) Sample Tubes .— 12 mm external diameter and 75 mm height.
( h ) Polypropylene Tubes .— 2 mL and 15 mL and 25 mL.
( i ) Frit filters .— Optional for matrices with a lot of particulate , CAT No. FRI001.
( j ) 70% v:v Ethanol .— For preparation of the Solus One Salmonella Supplement.
( k )
Deionized water .
( l ) Sterile collection sponge for sampling environmental surfaces .— World Bioproducts EZ Reach
Sponge Sampler with Hi Cap Neutralizing Buffer, or equivalent.
( m ) Sterile collection swab for sample environmental surfaces . ( n ) Letheen Broth neutralizing buffer .
Apparatus
( a ) Refrigerator .— capable of maintaining 2–8°C.
( b ) Incubators .— capable of maintaining 37 ± 1°C and 41.5 ± 1°C.
( c ) Circulating water bath .— capable of maintaining 42–50°C.
( d ) Heating block or water bath .— capable of maintaining 85–100°C.
( e ) Autoclave for decontamination of samples . ( f ) Measuring cylinder .— capable of measuring 2 L.
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