Total Collaborative Study Protocol_Solus One Salmonella v1 1
( b ) The National Collection of Type Cultures (NCTC) .— Salisbury, SP4 0JG, UK.
http://www.phe‐culturecollections.org.uk/collections/nctc.isp.
( c ) U.S. Food and Drug Administration Culture Collection (FDA) .— 10903 New Hampshire Avenue,
Silver Spring, MD, 20993, USA.
https://www.fda.gov
( d ) University of Pennsylvania Culture Collection (STs) .— Department of Microbiology, Perelman
School of Medicine, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia,
PA, 19104, USA.
( e ) Q Laboratories, Inc. Culture Collection (QL) .— Patrick Bird, Project Leader, Q Laboratories, Inc.,
Research and Development, Cincinnati, OH, 45214, USA.
Safety Precautions
Solus One Salmonella Method .— Ensure that the heating block reaches a temperature of 85–100°C
and the sample is heated for 15–20 min thus ensuring the organisms are killed and the sample is safe to
handle. The Stop Solution contains sulfuric acid which is corrosive. Wash immediately with large
quantities of water if the solution comes into contact with skin or mucous membranes. All other Solus
One Salmonella kit components are non‐hazardous. However, product usage should follow good
laboratory practices and all disposable materials must be discarded according to appropriate waste
procedures used in the laboratory. Protective clothing should be worn including laboratory coat, safety
glasses, mask, and gloves where appropriate. Avoid contact with the skin.
Enrichment .— Salmonella is a Biosafety Level 2 organism. Biological samples such as enrichments
have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or
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