Total Collaborative Study Protocol_Solus One Salmonella v1 1
( e ) Autoclave for decontamination of samples .
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( f ) Homogenizer
(g) Measuring cylinder .— capable of measuring 2 L. ( h ) Vessel or container .— capable of storing 2 L.
( i )
Filter laboratory blender bags .
( j ) Sterile collection sponge and swabs for sampling environmental surfaces . 6 ( k ) Serological Pipette Bulbs (Automatic Pipette) .— For sampling and delivering of 1–10 mL. 7 ( l ) Serological pipettes .— Aerosol resistant. 8 ( m ) Precision pipettes .— For sampling and delivering of 100–300 µL, and 200–1,000 µL. 9 ( n ) Micropipette tips .— Aerosol resistant. 10 ( o ) Multi‐channel pipette .— Capable of delivering 100–300 µL. 11 ( p ) Microplate washer . 12 ( q ) Microplate reader with 450nm filter . 13 ( r ) Calibrated Thermometers .— Capable of measuring a temperature of 37 ± 1°C, 41.5 ± 1°C, and 14 85–100°C. 15 ( s ) Vortex mixer .— For swab homogenization. 16 ( t ) Calibrated Timer . 17 ( u ) Marker .
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C. Media and Reagents
( a ) One or Five x 96 well microplates (in breakable strip format) .— Wells are coated with
antibodies against Salmonella species. Store at 2–8°C.
( b ) Negative Control (Green Label) .— 3 mL or 10 mL in working dilution. Contains diluent with
preservative. Store at 2–8°C.
( c ) Positive Control (Red Label) .— 3 mL or 10 mL in working dilution. Contains heat‐killed 26 Salmonella Agona in diluent with preservative. The positive control is black in color. Store at 2–8°C. 27 ( d ) Conjugate (Orange Label) .— 11 mL or 60 mL in working dilution. Contains horseradish 28 peroxidase‐antibody conjugate in diluent with preservative. Store at 2–8°C. 29 ( e ) Substrate (Blue Label) .— 11 mL or 60 mL in working dilution. Contains 3,3’,5,5’‐ 30 Tetramethylbenzidine (TMB), hydrogen peroxide and stabilizers. Note: Solution should be colorless. 31 Store at 2–8°C. 32 ( f ) Stop Solution (Silver Label) .— 11 mL or 60 mL in working dilution and contains 0.2M sulfuric 33 acid. Store at 2–8°C. 34 ( g ) Washing Buffer Concentrate (25x) .— 6 x 10 mL or 5 x 60 mL. Store at 2–8°C. 35 ( h ) Washing Buffer Activator .— 6 x 1 Sachet or 5 x 1 Sachet. Store at 2–8°C. 36 ( i ) Product Instructions .
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D. Additional supplies and reagents
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