Total Collaborative Study Protocol_Solus One Salmonella v1 1
Table 6: Robustness study of Solus One Salmonella Method, POD comparison
Parameters
Substrate incubation (min)
Parameter test combination a
Culture time (h)
Conjugate vol. (µL)
Nominal condition e
N b
x c
POD E
95% CI
N
x
POD N f
95% CI
dPOD EN
95% CI h
d
g
Target analyte: Raw beef (trim – >30% fat content) inoculated with Salmonella Typhimurium NCTC 5711 1 18 90 20 10 8 0.80 0.49, 0.94 9 10 8 0.80
0.49, 0.94 0.49, 0.94 0.49, 0.94 0.49, 0.94 0.49, 0.94 0.49, 0.94 0.49, 0.94 0.49, 0.94 0.49, 0.94 0.00, 0.28 0.00, 0.28 0.00, 0.28 0.00, 0.28 0.00, 0.28 0.00, 0.28 0.00, 0.28 0.00, 0.28 0.00, 0.28
0.00 0.00 0.00 0.00 0.10 0.10 0.10 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
‐0.34, 0.34 ‐0.34, 0.34 ‐0.34, 0.34 ‐0.34, 0.34 ‐0.24, 0.43 ‐0.24, 0.43 ‐0.24, 0.43 ‐0.24, 0.43 ‐0.34, 0.34 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28 ‐0.28, 0.28
2 3 4 5 6 7 8 9 2 3 4 5 6 7 8 9
18 18 18 26 26 26 26 20 18 18 18 26 26 26 26 20
90
40 20 40 20 40 20 40 30 40 20 40 20 40 20 40 30
10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
8 8 8 9 9 9 9 8 0 0 0 0 0 0 0 0
0.80 0.80 0.80 0.90 0.90 0.90 0.90 0.80 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.49, 0.49, 0.49, 0.60, 0.60, 0.60, 0.60, 0.49, 0.00, 0.00, 0.00, 0.00, 0.00, 0.00, 0.00, 0.00, 0.00,
0.94 0.94 0.94 1.00 1.00 1.00 1.00 0.94 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28
9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9
10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
8 8 8 8 8 8 8 8 0 0 0 0 0 0 0 0 0
0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
110 110
90 90
110 110 100
Non‐target analyte: Un‐inoculated raw beef (trim, >30% fat content) 1 18 90 20 10 0 0.00
90
110 110
90 90
110 110 100
a Each parameter test combination is being compared to the nominal test condition. b N = Number of test portions experimental combination. c x = Number of positive test portions experimental combination. d POD E = Positive outcomes divided by the total number of trials experimental combination. e Nominal condition = parameter test combination No. 9.
Page 43 of 47
Made with FlippingBook - Online Brochure Maker