Breckland Scientific 2023 Catalogue

Electrophoresis

UK made Electrophoresis Kits. These exciting kits come either with real DNA samples for those who prefer to see authentic banding using actual DNA. These kits do take longer than equivalent dye versions to complete as the gels will need staining and de-staining in order to visualise the DNA banding. Alternatively visualise the DNA kits under Blue light – see transilluminator Electrophoresis Kits

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1 CYSTIC FIBROSIS TESTING KIT Use this electrophoresis kit to teach genetic testing for cystic fibrosis, which is caused by a 3 base pair deletion and is easily seen using DNA electrophoresis. With this set, a mother, father and child are tested. Your students will see a normal, carrier and affected genotype in the control samples provided. You can use the set to teach the meaning of the terms heterozygote and homozygote. The ethical issues raised by genetic testing can also be covered. Additional equipment required for this DNA kit: Electrophoresis tank; power supply; fixed volume or adjustable 40µl micropipette and tips. Kit contains: instructions, DNA samples, DNA stain, agarose, electrophoresis buffer. For 8 gels/student groups. Cat No. Description Price Per BIO-520-100 Cystic Fibrosis DNA Testing Kit 80.25 kit 2 DNA FINGERPRINTING KIT Students can solve a crime with this exciting electrophoresis kit. They must match the DNA pattern from the crime scene with one of five suspects to find the culprit, by matching the banding pattern to work out the result. Additionally, advanced students can learn about short tandem repeats (STR) – a type of variable number tandem repeat (VNTR) using the kits. All you need: electrophoresis tank, power supply, fixed volume 3 PATERNITY TESTING KIT Paternity testing is now very routine around the world. Give your students an understanding of this technique with these exciting electrophoresis kits. Compare a child’s DNA with their mother and two possible fathers. Students must match the banding pattern to work out the result. Additionally, advanced students can learn about short tandem repeats (STRs) – a type of variable number tandem repeat (VNTR) using the kit. All you need: electrophoresis tank, power supply, fixed volume or adjustable 40µl micropipette and tips, plus the DNA kit. DNA kit includes: instructions, DNA samples, DNA stain, agarose, electrophoresis buffer for 8 gels. PLEASE NOTE: the DNA kit has a shelf-life of 6 months and will be ordered as required. Please use within 6 months of purchase. Cat No. Description Price Per BIO-540-100 Paternity Testing DNA Kit 80.25 kit 4 BREAST CANCER GENETICS KIT Breast cancer is the most common type of cancer, with around 5-10% of all breast cancers being inherited. Mutations in the breast cancer 1 (BRCA1) gene greatly increase the risk of developing breast and ovarian cancer. This gene is found on chromosome 17 and is normally responsible for DNA repair and is known as a tumour suppressor gene. Mutations in BRCA1 are dominant and so the presence of one copy of a mutated form can cause an increased risk of cancer. This experiment will test DNA of members of a family where the grandmother (generation 2) and her daughter (generation 3) both developed breast cancer - both had a mutation in their BRCA1 gene. Each kit includes enough DNA, agarose gel, electrophoresis buffer and DNA stain for up to 8 groups of students (8 gels). Also required is a horizontal electrophoresis system with power supply and pipettes. • Whole experiment takes less than 1 hour • Great for introducing genetic diseases and genetic screening to students • Works perfectly with Cleaver Scientific electrophoresis equipment Cat No. Description Price Per BIO-560-100 Breast Cancer Genetics Kit 75.97 kit or adjustable 40µl micropipette and tips, plus the DNA kit. The kit contains: instructions, DNA samples, DNA stain, agarose, electrophoresis buffer for 8 gels/student groups. Cat No. Description Price Per BIO-530-100 DNA Fingerprinting DNA Kit 82.75 kit

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Cystic Fibrosis Testing DNA Kit (Inset - DNA Kit as supplied)

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DNA Fingerprinting DNA Kit

Paternity Testing DNA Kit

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Breast Cancer Genetics Kit

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