SPDS 09052014

DRAFT AOAC SMPR 2014.XXX; Version 3; July 16, 2014 1 2 Method Name: 3 4 Purpose: AOAC SMPR’s describe the minimum recommended performance characteristics to be 5 used during the evaluation of a method. The evaluation may be an on-site verification, a single- 6 laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by 7 AOAC Stakeholder Panels composed of representatives from the industry, regulatory 8 organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC 9 SMPRs are used by AOAC Expert Review Panels in their evaluation of validation study data for 10 method being considered for Performance Tested Methods or AOAC Official Methods of 11 Analysis , and can be used as acceptance criteria for verification at user laboratories. Determination of Total Chondroitin Sulfate

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Approved by: Stakeholder Panel on Dietary Supplements (SPDS)

Final version date :

Effective date:

Intended Use : Reference method for dispute resolution.

1. Applicability :

Quantitative determination of total chondroitin sulfate salts in dietary ingredients and dietary

supplements.

2. Analytical Technique :

Any analytical technique(s) that measures the analytes of interest and meets the following method performance requirements is/are acceptable. It is acceptable to have a different

analytical method for each class of analytes.

3. Definitions :

Chondroitin Sulfate

Chondroitin Sulfate Sodium consists mostly of the sodium salt of the sulfate ester of N- acetylgalactosamine (2-acetamido-2-deoxy--d-galactopyranose usually abbreviated as GalNAc) and d-glucuronic acid copolymer. These hexoses are alternately linked -1,4 and -1,3 in the polymer. It is closely related to other GAGs such as dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and keratan sulfate which contain other hexosamine and/or glycuronic acid residues. Either of the residues can be sulfated at different positions.

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