SPDS 09052014

8/22/2014

Identity Methods for CS

– Enzymatic HPLC • CS is selectively digested by chondroitinase AC  enzyme into unsaturated disaccharides. • Unsaturated disaccharides can be quantified by  HPLC. • Related GAGs do not interfere with test. • Problems of availability for disaccharide standards  (esp. Δdi‐6S) and enzyme (new USP monograph has  addressed this issue by using well characterized  reference material verses individual disaccharides) • Requires experienced chemist to run.

Enzymatic-HPLC Analysis of Bovine CS

1 = Δdi‐0S 2 = Δdi‐4S 3 = Δdi‐6S

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