2015.14 Vitamin B (July 2018)

2015.14 (JULY 2018) BVit-02 Total B_AOAC_2015.14_MLT_Final

FOR ERP USE ONLY DO NOT DISTRIBUTE

[Vit] WSx :

Vitamin concentration in the working standard in ng/mL.

[Vit] MWS :

Concentration of vitamin in the Mixed Working Standard in ng/mL.

Vol MWS :

Volume of the Mixed Working Standard fortified in working standard in μL.

500:

This value is the dilution factor.

4. Vitamin concentration calculated in product from analytical result:

AS ] [

× × 500

RW Vit

sample ] [

Vit

=

PW SW

×

Where, [Vit] sample :

Vitamin concentration in product, μg/kg

[Vit] AS : Vitamin mass in the analytical sample as calculated from calibration curve, ng/mL. RW: Reconstitution weight (total), g. For direct weight (liquid) samples RW = 1. SW: Analytical sample weight, g. PW: Powder weight (for reconstituted samples), g. For liquid samples, this value is 1. 500: This value is the dilution factor.

5. For vitamins B 3 and B 6 , the reported concentration of the individual forms is summed to report total. For example, concentration of nicotinamide and nicotinic acid are summed to report “Total B 3 ” and concentration of pyridoxal, pyridoxamine, and pyridoxine are summed to report “Total B 6 .” Thiamine and Riboflavin don’t require this step.

References 1. AOAC SMPR 2015.002 (2015) J. AOAC Int. 98, 1094. 2. AOAC SMPR 2015.003 (2015) J. AOAC Int. 98, 1096. 3. AOAC SMPR 2015.004 (2015) J. AOAC Int. 98, 1098. 4. AOAC SMPR 2015.005 (2015) J. AOAC Int. 98, 1100.