Chemistry-PTM-OMA_Modules_1-4

Limit of Detection (LOD) and  Limit of Quantitation (LOQ) Estimate LOD and LOQ for each matrix one of several ways: 1. Plot s r vs. mean result from matrix study and perform regression a. Estimate LOD as                , where       is the mean value of blank replicates,      is the  intercept, and      is the slope b. Estimate LOQ as LOD x 3. 2. Analyze 10 blank test portions and 10 test portions containing a low    0 3.3 1 1.65 b X s m   b s m 0 X

concentration of analyte (near the expected LOQ) a. Estimate LOD as the mean blank result + 3 SD low conc b. Estimate LOQ as the mean blank result + 10 SD low conc 3. Analyze 10 blank test portions a. Estimate LOD as the mean blank result + 3 SD b. Estimate LOQ as the mean blank result + 10 SD