Chemistry-PTM-OMA_Modules_1-4

Data Table – Quantitative Method SLV Candidate Method Results for Histamine in Frozen Tuna Compared to a Known Spike Value

Test Portion

Spike  ppm

Result ppm

Spike  ppm

Result ppm

Spike  ppm

Result ppm

Spike  ppm

Result ppm

Spike  ppm

Result ppm

Spike ppm

Result ppm

1 2 3 4 5 6 7 8 9

0 0 0 0 0 0 0 0 0 0

0.0

2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5

2.1 2.3 2.1 2.4 2.1 2.3 2.2 2.3 2.1 2.3

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

5.4 5.1 4.8 5.3 5.2 4.9 4.9 5.6 5.0 5.2

10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0

10.5 10.2 11.0 11.5 10.1 12.1 10.8 10.5 11.1 10.8 0.69 9.8

20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0

24.4 19.7 24.0 22.4 20.2 21.0 22.9 23.6 21.4 23.0 22.3 1.62

50.0 50.0 50.0 50.0 50.0 50.0 50.0 50.0 50.0 50.0

49.5 57.3 51.2 54.9 58.9 59.4 53.6 55.0 56.3 51.7 54.8 3.31

‐0.1 0.0 0.3 0.1 0.2 0.3 0.2 0.3 ‐0.1

10

Mean 0.12

Mean

2.22 0.11

Mean

5.14 0.25

Mean

Mean

Mean

s r

0.16

s r

s r

s r

s r

s r

RSD r 6.04% Recovery 88.8% Recovery 102.8% Recovery 108% Recovery 111.3% Recovery 109.6% Bias ‐0.28 Bias 0.14 Bias 0.8 Bias 2.3 Bias 4.8 4.95% RSD r 4.86% RSD r 6.39% RSD r 7.26% RSD r