2018 March 16 SPDS

Method Performance Requirements: Kava

Applicability: Identification and quantitation of the six major kavalactones and flavokavains A, B, and C derived from the underground portions of kava ( Piper methysticum ) in plant material, dietary ingredients and dietary supplements (dried plant material, liquid extracts including tinctures, soft extracts, dry extracts, tablets, and capsules including softgels).

Kavalactones* Flavokavains*

Analytical Range (mg/g)** Limit of Quantitation (mg/g) 

5 – 750

0.1 – 25

≤ 5

≤ 0.1

*Reported as individual constituents. **Range may be narrower depending on the analytical matrix.

Method Performance Requirements: Kava

Method Performance Requirements as a Function of Range

Acceptance  Criteria Lower Range  Flavokavains* 

Acceptance  Criteria  Upper Range  Flavokavains * 

Acceptance  Criteria  Kavalactones* 

Parameter

% Recovery

90 – 110 

90 – 110

% RSD r (repeatability)

≤ 7.5

≤ 15

≤ 7.5

≤ 10

% RSD R (Reproducibility)

≤ 10

≤ 20

*Reported as individual constituents.