September 2016 SPDS Book

General Analytical Needs

 Method should ▪ Separate and accurately determine both vitamin K1 (phylloquinone) and K2 (different menaquinones) ▪ Determination of trans -K1 and cis -K1 (defined as the sum of cis and trans isomer of K1) ▪ Separate and accurately determine three different forms of K2 (MK4, MK6 and MK7) ▪ Determination of all- trans -MK4, all- trans MK6 and all- trans MK7. Many cis forms maybe present. ▪ Be able to analyze both coated and non-coated formulations ▪ Determine the above in raw materials used to produce/formulate dietary supplements

Challenges

▪ Need cost effective, quick turnaround analysis time and one test method that can be run in an in-house laboratory. ▪ Vitamin K is unstable to light, alkali and oxidizing agents ▪ Must be protected from light ▪ Use of non-UV lamps ▪ Brown glassware