September 2016 SPDS Book

Background

Summary of current methods used in Aloe  composition analysis:

• NMR ‐ quantification of polysaccharides and  providing aloe fingerprint for identification; – Quantification of polysaccharides by analysis of the  content of acetyl groups; – Quantification of organic acids including acetic acid, lactic  acid,  malic acid and isocitric acid;  • GC – quantification of aloe organic acid, such as acids  of lactic, malic, citric, isocitric, etc.; quantification of  monosaccharides including existed monosaccharides  or hydrolyzed from polysaccharides;

Background

Summary of current methods used in Aloe  composition analysis ( cont’d ): • Colorimetric – quantification of aloe polysaccharides  by photometric analysis;   • GPC – providing fingerprint of aloe polysaccharides  and their molecular weight and size; • HPLC – quantification of organic acids, including  oxalic, malic, isocitric, lactic, citric, fumaric acid, and  isocitric acid lactone; providing aloe fingerprint for  identification;