Centrifugation Application Notes
Complete Solutions for Your Specific Exosome Workflow – from Preparation to Analysis
Beckman Coulter provides solutions customized for each step in your specific exosome research workflow – from sample preparation to sample analysis – and can also help improve processes and assist with related challenges.
Sample Preparation
Solution
Step
Jurkat cells grown in log phase density - 1 x 1 0 6 cells/mL
1
Vi-CELL XR
2 Centrifugation to remove cells and debris
Allegra X- 1 5R
3 Ultracentrifugation to remove smaller cellular debris
Optima XPN
This chart provides a sample workflow and the solution for each step.
4 Ultracentrifugation to pellet exosomes
Optima XPN
5 Automated method for density gradient set-up
Biomek 4000Workstation
6 Density gradient ultracentrifugation to isolate exosome from co-purified proteins and other membrane vesicles
Optima XPN
7 Ultracentrifugation to exchange solvent from Iodixanol and sucrose to phosphate buffered saline
Optima MAX-XP
Step Sample Analysis
Solution
8 Size determination and analysis
DelsaMax CORE
Contact your Beckman Coulter Life Sciences Sales Representative for options to improve your processes and to address your specific exosomes workflow:
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