Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD29 The CD29 antigen is the 130 kDa integrin β 1 chain which is expressed as a heterodimer, non-covalently associated with the integrin α subunits 1 to 6 (CD49a to CD49f) also known as Very Late Antigens (VLA). CD29 complexes are involved in cell-cell and cell-matrix adhesion, depending on the α subunit associated to CD29. CD29 is also known as platelet GPIIa. The antigen mediates cellular adhesion and has broad cellular reactivity, but is not expressed by erythrocytes. In normal peripheral blood it is found on a subset of CD4+ and CD8+ T lymphocytes, on a minority of B lymphocytes and on monocytes. The "inducer" subpopulation of CD4+ lymphocytes co-expresses CD4 and CD29.

Clone: 4B4LDC9LDH8 (4B4) Isotype: IgG1 Mouse 4B4LDC9LDH8 is a blocking monoclonal antibody in CD29 mediated adhesion. This antibdy was used as CD29 reference monoclonal antibody during HLDA 6.

Form

Size

Format

Part#

Status

Line Quality Standard

RD1 RD1

6603177 6604159 6603113

100 tests 50 tests

Liquid Liquid

RUO RUO RUO

GMP GMP GMP

COULTER CLONE

CYTO - STAT

UNLB

Freeze-dried 100 tests

COULTER CLONE

CD31 The CD31 antigen, also known as Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a transmembrane glycoprotein of 130 kDa related to the immunoglobulin superfamily. It is expressed on stem cells of the myeloid lineage, on platelets and on endothelial cell junctions. CD31 is involved in the migration of leucocytes through the endothelial cell wall, via adhesion to α v β 3 integrin and to CD38. In addition, CD31 participates in outside-in signaling in leucocytes likely through phosphorylation of its intracytoplasmic tyrosine residues 663 and 686 and subsequent association with tyrosine phosphatases SHP-1 and SHP-2. Studies suggest a comparable mechanism of action for CD31 in activation and aggregation of platelets.

Form Clone: 1F11

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM2409

100 tests

Liquid

RUO

IOTest

GMP

CD34 The CD34 antigen is a monomeric transmembrane phosphoglycoprotein of about 110 kDa with two distinct extracellular domains. The membrane proximal domain, of about 110 amino acids, probably adopts a globular conformation. The NH2- terminal domain, of about 140 amino acids, is heavily glycosylated with both N-linked glycans and sialylated O-linked carbohydrates and probably exhibits an extended rod-like structure typical of mucin-like glycoproteins. The CD34 antigen is expressed on hematopoietic progenitor cells of all lineages as well as the most primitive pluripotential stem cells. CD34 antigen expression is highest on the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. Variations of glycosylation are thought to occur during normal hematopoiesis depending on lineage commitment and the level of cellular maturation.

Clone: Immu133

Isotype: IgG1 Mouse

Form Epitope Class I : Immu133 monoclonal antibody detects sialic acid-dependent class I epitope. Part# Format Size Status

Line Quality Standard

PE

IM1420

100 tests

Liquid

RUO

IOTest

GMP

Form Isotype: IgG1 Mouse Epitope Class II: QBEnd10 reacts with early normal hematopoietic progenitor cells, and with vascular endothelial cells. Part# Format Size Status Line Quality Standard UNLB - RUO IM0786 Freeze-dried 0.2 mg GMP Clone: QBEnd10

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 179 US Research Flow Cytometry Catalog