Expert Review Panel for Dietary Supplements

AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM

Submission Date

2017-12-07 16:31:32

AOAC Expert Review Panel: Method Review

Reviewer Information Required to validate your review.

Name

Garrett Zielinski

E-mail

Garrett.Zielinski@covance.com

Organization

Covance Laboratories

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Free Amino Acid Profile and Absence Verification Method by UPLC

AOAC Candidate Method Number (e.g. ALN-01)

FAA-001

Applicable SMPR

2017.011

I. Summary of the Method

I. Summary of the Method

Samples are dissolved in a mixture of water:6M HCl and internal standard is added. An aliquot is diluted with purified water and derivitized with AQC reagent. Analytical separation is performed via UPLC with a C18 column. Detection and quantitation are performed via absorbance at 260nm and comparison to standards of known concentrations.

II. Review of the Method Only: