Expert Review Panel for Dietary Supplements

AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM

Submission Date

2017-12-12 13:01:17

AOAC Expert Review Panel: Method Review

Reviewer Information Required to validate your review.

Name

SNEH BHANDARI

E-mail

sneh.bhandari@mxns.com

Organization

Merieux NutriSciences

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Identification of Pea, Rice and Soy Proteins in Raw Materials and ------------------ --HPLC-MS/MS

AOAC Candidate Method Number (e.g. ALN-01)

PRO-001

Applicable SMPR

AOAC SMPR 2016.016

I. Summary of the Method

I. Summary of the Method

The samplle is extracted in 5 mM Urea Tris buffer followed by reduction with DTT and alkylation with iodoacetamide. The extract is digested with trypsin at 37C overnight abd quenched by formic acid.The extract is filtered and diluted with 0.1% formic acid and internal std (Beta-Casomorphin 1-4 peptide) and analyzed by LC-MS/MS. Pea, rice and soy protein identifications were based on the comparison with MRL sample, which is made from raw materials identified using PCR.

II. Review of the Method Only:

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