Expert Review Panel for Dietary Supplements

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). III. Review of Supporting Information 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference.

Yes, with scope of Pea, Rice and Soy proteins in raw and finished dietary supplement materials

Yes. Pea, rice and soy protein identifications in the method based on the comparison with MRL sample, which is made from raw materials identified using PCR. The identification is LC-MS/MS based by analysis of corresponding specific peptides in the samples following tryptic digestion of the extracted proteins.

Yes,

No distinctly. The authors may add a separate paragraph about safety emphasizing toxicity of reagents like iodoacetamide and other chemicals

III. Review of Information in Support of the Method

Yes.