Expert Review Panel for Dietary Supplements

AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM

Submission Date

2017-12-08 04:08:02

AOAC Expert Review Panel: Method Review

Reviewer Information Required to validate your review.

Name

Hong You

E-mail

hongyou@eurofinsus.com

Organization

Eurofins Scientific, Inc.

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Identification of Milk Proteins in Raw Materials and Finished Goods using ESI HPLC-MS/MS

AOAC Candidate Method Number (e.g. ALN-01)

PRO-002

Applicable SMPR

2016.015

I. Summary of the Method

I. Summary of the Method

This method is applicable for the identification of milk proteins (a-S1-Caein and b-Lactoglobulin) in dietary supplement raw materials and finished products. The proteins in sample were treated with urea and fractionated by trypsin into specific peptides, which are then detected by HPLC-triple quad mass spectrometry. Three peptides were chosen as target analytes for the identification of each protein.

II. Review of the Method Only: