Expert Review Panel for Dietary Supplements

AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM

Submission Date

2017-12-13 15:36:52

AOAC Expert Review Panel: Method Review

Reviewer Information Required to validate your review.

Name

Philip Haselberger

E-mail

philip.haselberger@abbott.com

Organization

Abbott Nutrition

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Identification of Milk Proteins in Raw Materials and Finished Goods using ESI HPLC-MS/MS

AOAC Candidate Method Number (e.g. ALN-01)

PRO-002

Applicable SMPR

2016.015

I. Summary of the Method

I. Summary of the Method

Method is based on an UPLC-MS/MS analysis of peptides after the sample has been through a trypsin digestion. Positive ID of the presence of milk protein at >0.1% is made by comparison relative to a lab prepared MRL sample that is nominally 0.1%. The presence of milk protein is only confirmed if 3 signature peptides from alpha-S1-casein and beta-lactoglobulin are all present. Otherwise the protein is not confirmed.

II. Review of the Method Only: