Expert Review Panel for Dietary Supplements

AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM

Submission Date

2017-12-08 15:19:13

AOAC Expert Review Panel: Method Review

Reviewer Information Required to validate your review.

Name

Catherine Rimmer

E-mail

catherine.rimmer@nist.gov

Organization

NIST

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Determination of Total Phenolic Content Using the Folin C Assay: Single Laboratory Validation

AOAC Candidate Method Number (e.g. ALN-01)

FOL-04

Applicable SMPR

2015.009

I. Summary of the Method

I. Summary of the Method

An aqueous extraction of a sample matrix is performed then followed by reaction with Folin & Ciocalteu's (Folin-C) reagent. The light absorption is then determined at 765 nm and compared with a gallic acid calibration curve. For this method, the procedure was examined in a series of dietary supplements including: grape seed extract, grape skin extract, black tea extract, green coffee extract and cocoa extract.

II. Review of the Method Only: