Microsoft Word - Draft OMB Meeting Agenda-April 11 2019

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OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

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h) Sterile collection sponge and swab . – Environmental surface sampling i) Thermometer . – Calibrated range to include 100 ± 1°C range j) Incubators . – Capable of maintaining 42 ± 0.2 ° C, 42 ± 0.5 ° C , 42±1°C , 36 ± 1 ° C, 35 ± k) Dry Bath Incubator . – Capable of maintaining a temperature of 100 ± 1 ºC l) Dry double block heater unit. – Capable of maintaining 100 ± 1°C; or a water bath m) Refrigerator . – capable of maintaining 2-8°C, for storing the 3M MDA2 n) Computer . – compatible with the 3M Molecular Detection System (instrument) 2°C, 35 ± 1.0 ° C, 43 ± 0.2 o C and 41.5 ± 1°C capable of maintaining 100 ± 1°C

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Safety Precautions

The 3M™ Molecular Detection Instrument is intended for use with samples that have undergone heat treatment during the assay lysis step, which is designed to destroy organisms present in the sample. Samples that have not been properly heat treated during the assay lysis step may be considered a potential biohazard and should NOT be inserted into the 3M Molecular Detection

Instrument.

The user should read, understand and follow all safety information in the instructions for the 3M Molecular Detection System and the 3M MDA 2 – Salmonella . Retain the safety instructions for

future reference.

Sample Preparation

3M recommends the use of 3M BPW ISO for the enrichment of food and environmental samples. Table 1 presents guidance for the enrichment of food and environmental samples. It is the user’s responsibility to validate alternate sampling protocols or dilution ratios to ensure this

test method meets the user’s criteria.

Foods

a) Allow 3M BPW ISO enrichment medium to equilibrate to ambient laboratory temperature or

AOAC Research Institute Expert Review Panel Use Only

pre-warm to 41.5 C depending upon matrices tested. See Table 1.

b) Aseptically combine the enrichment medium and sample. For all meat and highly particulate

samples, the use of filter bags is recommended. c) Homogenize thoroughly for 2 ±0.2 minutes.

Environmental samples

Sample collection devices can be a sponge hydrated with a neutralizing solution to inactivate the effects of the sanitizers. 3M recommends the use of a biocide-free cellulose sponge. Neutralizing solution can be Dey-Engley (D/E) Neutralizing Broth and Letheen. It is recommended to

sanitize the area after sampling.

WARNING: Should you select to use Neutralizing Buffer (NB) that contains aryl sulfonate complex as the hydrating solution for the sponge, it is required to perform a 1:2 dilution (1 part sample into 1 part sterile enrichment broth) of the enriched environmental sample before testing in order to reduce the risks associated with a false-negative result

04/05/2019