OMB Meeting Book_9-11-14

ISO/WD

Table B.10 – Calculation of fatty acids (Example using Excel)

1

2

Response Repetition number

Results expressed in

x

x

factor

File name

gFA/100g

xx-xx-xx xx-xx-xx

related to Injection date

gFA/100g

301.80 285.00 total FA

Test portion (mg) product or oil

C-11:0

product (or oil)

9.620 9.6200

internal

C-11:0 FAME Internal standard amount (mg)

(FA%)

101400 101509 Mean 1

2 Mean SD RSD

standard C-11:0 FAME Internal standard area

9.375

(n=2)

(n=2)

9.375

%

C-13:0 (TAG) Internal standard amount (mg)

104944 105005

C-13:0 (TAG) Internal standard area

0 0.000

0.000 0.000

0.000 #DIV/0!

0

0.000

1.668 C-4:0

butyric

990 0.154

0.038 0.038

0.000 0.0

1047

0.038

1.282 C-6:0

caproic

0.377 0.377

0.000 0.0

11737 11100 1.537

0.377

1.121 C-8:0

caprylic

9780 1.248

0.305 0.306

0.001 0.2

10317

0.306

1.018 C-10:0

capric

2.157 2.164

0.006 0.3

76477 72560 8.816

2.160

0.959 C-12:0

lauric

0.961 0.965

0.002 0.2

35347 33520 3.930

0.963

0.919 C-14:0

myristic

180 0.022

0.005 0.005

0.000 1.0

193

0.005

0.939 C-14:1

myristoleic

N-5 (or w 5)

D9

620 0.073

0.018 0.018

0.000 0.0

656

0.018

0.915 C-15:0

pentadecanoic

0 0.000

0.000 0.000

0.000 #DIV/0!

0

0.000

0.927 C-15:1

pentadecenoic

N-5 (or w 5)

D10

5.953 5.982

0.021 0.3

223376 212207 24.352

5.968

0.894 C-16:0

palmitic

1840 0.216

0.053 0.053

0.000 0.0

1945

0.053

0.912 C-16:1

palmitoleic

N-7 (or w 7)

D9

840 0.096

0.024 0.024

0.000 0.1

890

0.024

0.887 C-17:0

margaric

259 0.030

0.007 0.007

0.000 0.3

275

0.007

0.896 C-17:1

heptadecenoic

0.934 0.939

0.003 0.3

35849 34052 3.822

0.937

0.870 C-18:0

stearic

1067 0.122

0.030 0.030

0.000 0.0

1128

0.030

0.881 C-18:1 TFA

total trans C18:1

8.706 8.749

0.030 0.3

334633 317905 35.613

8.727

0.868 C-18:1

oleic & others cis

N-9 (or w 9)

D9

2513 0.284

0.069 0.070

0.000 0.4

2642

0.070

0.878 C-18:2 TFA

total trans C18:2

3.802 3.822

0.014 0.4

146659 139350 15.555

3.812

0.866 C-18:2

linoleic

N-6 (or w 6)

D9,12

310 0.035

0.008 0.009

0.000 0.6

325

0.008

0.866 C-18:2 CLA N-7 (or w 7)

conjugated linoleic

D 9c/11t

232 0.026

0.006 0.006

0.000 1.0

242

0.006

0.881 C-18:3

gamma-linolenic

N-6 (or w 6)

D6,9,12

2040 0.228

0.056 0.056

0.000 0.4

2146

0.056

0.869 C-18:3 TFA

total trans C18:3

0.414 0.416

0.001 0.3

15922 15120 1.693

0.415

0.869 C-18:3

alpha-linolenic

N-3 (or w 3)

D9,12,15

3210 0.357

0.087 0.088

0.000 0.1

3390

0.087

0.857 C-20:0

arachidic

3620 0.406

0.100 0.099

0.000 0.1

3833

0.099

0.863 C-20:1

eicosenoic

N-9 (or w 9)

D11

245 0.027

0.007 0.007

0.000 1.7

253

0.007

0.861 C-20:2

eicosadienoic

N-6 (or w 6)

D11,14

222 0.025

0.006 0.006

0.000 1.5

230

0.006

0.868 C-20:3

eicosatrienoic (DHGLA)

N-6 (or w 6)

D8,11,14

0 0.000

0.000 0.000

0.000 #DIV/0!

0

0.000

0.868 C-20:3

eicosatrienoic

N-3 (or w 3)

D11,14,17

2385 0.267

0.065 0.066

0.000 0.6

2502

0.065

0.866 C-20:4

arachidonic (AA or ARA)

N-6 (or w 6)

D5,8,11,14

686 0.080

0.020 0.019

0.000 2.1

748

0.020

0.887 C-20:5

eicosapentanoic (EPA)

N-3 (or w 3)

D5,8,11,14,17

1709 0.189

0.046 0.047

0.000 0.7

1791

0.046

0.854 C-22:0

behenic

1234 0.139

0.034 0.034

0.000 0.7

1293

0.034

0.870 C-22:1

erucic

N-9 (or w 9)

D13

0 0.000

0.000 0.000

0.000 #DIV/0!

0

0.000

0.854 C-22:2

docosadienoic

N-6 (or w 6)

D13,16

1975 0.226

0.056 0.055

0.000 0.8

2112

0.055

0.875 C-22:6

docosahexaenoic (DHA)

N-3 (or w 3)

D4,7,10,13,16,19

1045 0.114

0.028 0.028

0.000 0.7

1095

0.028

0.839 C-24:0

lignoceric

395 0.044

0.011 0.011

0.000 1.7

408

0.011

0.869 C-24:1

nervonic

N-9 (or w 9)

D15

2415 0.274

0.067 0.067

0.000 0.5

2537

0.067

0.882 OFA

other fatty acids

921998 875626

100.000 24.450 24.562 24.506

0.079 0.3

Total

0.155 0.156

0.001 0.3

Total trans fatty acids

0.634

0.155

99.3 99.3

0.0 0.0

Transesterification performance (minimum 98.0 %)

99.3

26

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